| 美國紫外交聯(lián)儀庫存庫號M216912 美國紫外交聯(lián)儀庫存庫號M216912 特點： 為了使核苷酸固定在膜上，傳統(tǒng)的方法是將膜置于真空烘箱中80度烘2小時，在紫外交聯(lián)儀上僅需30-60秒 與真空烘焙相比，大大增加雜交信號強(qiáng)度 內(nèi)部配備光度檢測儀，可以補(bǔ)償由于紫外燈泡老化造成的輸出偏移 具自動交聯(lián)、時間和能量三種模式供 帶有顯示時間、能量的及設(shè)置面板
應(yīng)用： 將核酸共價結(jié)合到固相支持物（如纖維素膜）上 Northem,Southern,dot及slot印跡分析 雜交芯片的制備 菌落、噬菌斑篩選，瓊脂糖凝膠中DNA的切割 產(chǎn)生胸腺二聚體，用于部分限制性內(nèi)切酶消化作圖 紫外敏感性測試，用于鑒定recA宿主菌 樣品紫外照射處理 誘導(dǎo)培養(yǎng)細(xì)胞的凋亡 照射波長：254nm(配8瓦254nm燈泡5個) 內(nèi)部尺寸：47.7W/34.3D/16.8H
Stratagene UV STRATALINKER 2400 UV Crosslinker
Agilent Genomics / Stratagene Stratalinker 2400 UV Crosslinker Features For Crosslinking DNA or RNA to Coated Glass Slides, Nylon, Nitrocellulose or Nylon-Reinforced Nitrocellulose Membranes Greatly Reduces Valuable Time From Several Oven Baking Hours to 30-60 Seconds Increases Hybridization Signal Compared to Oven Baking Internal Photodetector Compensates for Shifting Output of Aging UV Bulbs Can be Operated in Autocrosslink, Time or Energy Modes
Description The Agilent Genomics / Stratagene UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25-50 seconds, in contrast to the traditional method of baking filters at 80for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal crosslinking performance, each Stratalinker UV crosslinker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs.
The Stratalinker UV crosslinker may be used for Northern, Southern, dot or slot blot analysis,1-3 colony or plaque screening, nicking of DNA in agarose gels prior to blotting,4 dimer formation to perform partial digests for rapid restriction mapping,5 UV sensitivity testing for host strain verification,6 and UV irradiation of PCR samples.7 The Stratalinker UV crosslinker may be used in the Autocrosslink, Time, or Energy modes, and the apparatus is available in two convenient sizes-the Stratalinker 2400 UV crosslinker is for larger membranes.
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